Select “Filter query for low complexity regions” Program: blastn (nucleic acid sequences), Database: nr (non-redundant), Make sure that the following are selected: Click on the accessory application menu,īLAST, NCBI BLAST over the internet. Select your sequence by clicking on the name. For further information, see the references in the library or the BioEdit Internet-based searches of the NCBI databases. The best matches are then output as aīioEdit does BLAST searches using a program that allows
If a match is found, the alignment isĮxtended in both directions for as long as the sequence matches. Sequence up into short “words” and comparing these with known sequences. Open BioEdit, click on the FileĮdited forward sequence file from last week.īLAST (Basic Local Alignment Search Tool) is a way ofĬomparing a nucleic acid or protein sequence to a database of known Log in to the computer using the same login and password as
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